The development of the detection kits arose from a conceptualized research program on molecular hybridization for rapid detection of economically-important microorganisms by a group of BIOTECH researchers. The R & D Projects for the PCR-based detection of E. coli, Salmonella, Staphylococcus aureus and Listeria spp. were then implemented with support from DOST, in addition to BIOTECH Core funding. The Salmonella DAS™ and Staphylococcus aureus DAS™ kits were each granted a patent on nucleic acid sequences and its use for detection by the Intellectual Property Office of the Philippines.