DNA Amplification System™ (DAS™) detection kits

DAS™ are rapid kits that utilize Polymerase Chain Reaction (PCR) technology for the detection of bacterial pathogens in food, water and animal feeds.

Quantity: 30 or 100 reaction tubes/pack

Storage: -20oC freezer

Shelf-life: 6 months

Precaution: Avoid exposure to high temperature and direct sunlight



How was the PCR-based DAS™ detection kits developed?

The development of the detection kits arose from a conceptualized research program on molecular hybridization for rapid detection of economically-important microorganisms by a group of BIOTECH researchers. The R & D Projects for the PCR-based detection of E. coli, Salmonella, Staphylococcus aureus and Listeria spp. were then implemented with support from DOST, in addition to BIOTECH Core funding. The Salmonella DAS™ and Staphylococcus aureus DAS™ kits were each granted a patent on nucleic acid sequences and its use for detection by the Intellectual Property Office of the Philippines.

What are the benefits of using DAS™ kit?
  • Next-day results. It is faster than the conventional method which normally takes 3-5 days for confirmed tests
  • Pathogen specific, accurate and highly sensitive
  • Results are definitive so there is no need for confirmatory tests
  • Easy to use and applicable in analyzing bulk
How do you use DAS™ kits?

Steps involved:

a) Sample enrichment in the recommended medium
b) Collection, washing and lysing of cells
c) Addition of cell lysates to the DAS™ reaction tubes
d) DNA amplification in the thermal cycler machine
e) Agarose gel electrophoresis and viewing of products with UV light


The Director UPLB-BIOTECH biotech.uplb@up.edu.ph